 |
분석 방법은 칩 종류와(Custom Methylation, cancer panel, Infinium HumanMehtylation27) 상관없이 동일한 과정을 거칩니다.
|
|
|
| Task |
Details |
| Raw data output |
Raw data files (experimental data processing) |
Explorer data:
correlation matrix, scatter plot, distribution summary , boxplot,
sample wise mclustering |
| Annotation information |
| Pre-processing |
Normalization (background, average) |
| Filtering (filtering data based on quality
assessment) |
| Statistical test |
Select significant CpG site and:
T-test, ANOVA, logistic regression, repeated measure anova, etc |
Multiple testing:
P-value correction (FDR, bonferroni, etc.) |
Classified into promter/
Non promoter region and hyper methylated, hypo methylated site |
| Clustering and heat map |
SHierarchical and K-means |
| Functional analysis |
Gene classification: biological process,
molecular function |
| Visualization |
Pathway, Venn-diagram |
| Result report |
Write the final result report |
|
|
