| Illumina expression beadchip workflow |
| 1. RNA Quality Check |
We perform the quality control of total RNA using
Agilent Bioanalyzer 2100 and NanoDrop spectrophotometer |
| 2. Sample Amplification and Labeling |
Amplification and labeling is performed using
the Ambion illumina RNA amplification kit |
| 3. BeadArray Hybridization |
Hybridization is performed using illumina expression beadchip kit |
| 4. Wash / Staning |
Washing is performed using illumina expression beadchip washing buffer kit |
| 5. Scan on BeadArray Reader |
Arrays were scanned with an illumina bead array Reader confocal scanner |
| 6. Analyze Data |
Array data export processing and analysis was performed using
illumina Beadstudio (Gene Expression Module) |
